Key Features
- Modified Sabouraud liquid formulation
- Designed for antibiotic potency determination
- Supports fungi and yeast growth
- Acidic pH reduces bacterial interference
- Referenced to USP standards
- Dehydrated powder for convenience and stability
Applications
- Microbiological antibiotic potency assays
- Fungal and yeast-based bioassays
- Quality control testing in pharmaceutical laboratories
✅ Product Specifications
- Product Name: Antibiotic Medium No. 13
- Alternative Name: Modified Sabouraud Liquid Broth
- Form: Dehydrated powder
- Package Size: 250 g / bottle
- Storage Condition: Room temperature
- Shelf Life: 3 years
- Reference Standard: USP
- Final pH (25°C): 5.6 ± 0.1
✅ Composition (per liter)
| Component | Quantity |
|---|---|
| Peptone | 10.0 g |
| Dextrose | 20.0 g |
✅ Preparation Instructions
- Suspend 30.0 g of the product in 1,000 mL of distilled or deionized water.
- Heat with agitation until completely dissolved.
- Dispense into suitable containers.
- Sterilize at 121°C for 15 minutes or 115°C for 30 minutes.
- Mix well after sterilization and allow to cool before use.
✅ Quality Control (QC)
Physical and Chemical Characteristics
| Test Item | Specification | Result |
|---|---|---|
| Powder Appearance | Light yellow powder, homogeneous, no impurities | Conforms |
| Solution Appearance | Light yellow, clear, no impurities | Conforms |
| pH (25°C) | 5.5 – 5.7 | 5.59 |
| Moisture | ≤ 5% | 3.2% |
Growth Performance (25°C, 7 days)
| Test Organism | Growth | Result |
|---|---|---|
| Aspergillus niger | Good growth | Pass |
| Candida albicans | Good growth | Pass |
| Saccharomyces cerevisiae | Good growth | Pass |