Ammonium Dextrose Test Agar for Bacterial Metabolism

Definitive Medium for Ammonium Utilization Testing
This specialized agar detects bacterial ability to use inorganic ammonium as sole nitrogen source through glucose metabolism. Bromothymol Blue indicator provides clear blue-to-yellow color transition.

Key Features:

• Low glucose concentration (2g/L) prevents false positives

• Optimized ammonium-phosphate buffer system

• Room temperature stable (3-year shelf life)

• Pre-measured components for batch consistency

Technical Specifications:
▶ Formula (per liter):
NaCl 5.0g | MgSO₄ 0.2g | NH₄H₂PO₄ 1.0g
K₂HPO₄ 1.0g | Dextrose 2.0g | BTB 0.08g | Agar 15.0g
▶ pH: 6.8±0.2 (25°C)
▶ Preparation: 24.2g/L → boil → sterilize at 121°C/15min

Critical Applications:

• Differentiation of Pseudomonas species

• Identification of Enterobacteriaceae

• Microbial nitrogen metabolism studies