Amies Transport Medium with Charcoal Powder

Amies Transport Medium with Charcoal is a dehydrated, semi-solid transport medium designed for the collection, transport, and short-term preservation of pathogenic microorganism specimens.

The inclusion of neutral activated charcoal helps absorb toxic metabolic by-products and inhibitory substances, improving the survival of fastidious organisms during transport from sampling sites to microbiology laboratories.

🔬 Intended Use

• Collection of pathogenic microorganism specimens

• Transport and preservation of clinical and environmental samples

• Maintenance of microbial viability during transport

📦 Product Specifications

• Product Name: Amies Transport Medium with Charcoal

• English Name: Amies Transport Medium with Carbon

• Physical Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature (RT)

• Shelf Life: 3 years

🧪 Medium Composition (per liter)

• Charcoal (neutral activated carbon) ………. 10.0 g

• Sodium chloride …………………………… 3.0 g

• Sodium hydrogen phosphate ……………… 1.15 g

• Potassium dihydrogen phosphate ………… 0.2 g

• Potassium chloride …………………………. 0.2 g

• Sodium thioglycollate ………………………. 1.0 g

• Calcium chloride ………………………….. 0.1 g

• Magnesium chloride ……………………….. 0.1 g

• Agar ………………………………………… 7.5 g

Final pH: 7.3 ± 0.1 (25 °C)

⚗️ Preparation Instructions

1. Suspend 23.3 g of the powder in 1 L of distilled or deionized water.

2. Heat to boiling with stirring until completely dissolved.

3. Dispense while stirring to ensure uniform distribution.

4. Sterilize at 121 °C for 15 minutes.

5. Cool to 50–55 °C, gently mix to evenly distribute charcoal.

6. Rapidly cool in cold water, allow to solidify, and store until use.