Actinomycete Broth is a microbiological culture medium developed for the cultivation of actinomycetes and related microorganisms in laboratory environments. The medium provides a nutrient-rich formulation suitable for microbial growth and metabolism during microbiological culture procedures.
Supplied as a dehydrated powder culture medium, Actinomycete Broth allows convenient preparation in microbiology laboratories. After dissolution and sterilization, the medium can be used as a liquid culture medium, or agar may be added to prepare semi-solid or solid culture media depending on experimental requirements.
This medium is commonly used in environmental microbiology laboratories, soil microorganism research, and academic studies involving actinomycetes and Streptomyces species.
Key Features
- Designed for actinomycetes cultivation
- Suitable for Streptomyces culture studies
- Microbiological broth culture medium
- Supplied as dehydrated powder
- Can be prepared as liquid, semi-solid, or solid medium
- Easy preparation and sterilization
- 250 g laboratory packaging
- Stable formulation with 3-year shelf life
Applications
- Cultivation of actinomycetes
- Streptomyces microbial culture
- Environmental microbiology research
- Soil microorganism studies
- Academic microbiology laboratories
Medium Composition
Composition (per liter)
Pancreatic Digest of Casein — 17.0 g
Heart Muscle Infusion from Solids — 2.0 g
Yeast Extract — 10.0 g
Sodium Chloride — 5.0 g
Dextrose — 5.0 g
Monopotassium Phosphate — 13.0 g
Monopotassium Phosphate — 2.0 g
Ammonium Sulfate — 1.0 g
L-Cysteine HCl — 1.0 g
Soluble Starch — 1.0 g
Magnesium Sulfate — 0.2 g
Calcium Chloride — 0.01 g
Final pH: 7.3 ± 0.2 (25°C)
Directions for Use
- Suspend 57.0 g of the medium in 1 liter of distilled or deionized water.
- If required, add 7 g/L or 20 g/L agar to prepare semi-solid or solid agar medium.
- Heat to boiling until the medium is completely dissolved.
- Dispense into suitable containers.
- Sterilize by autoclaving at 121°C for 10 minutes.
- After sterilization, mix thoroughly and allow to cool before use.
Product Specifications
| Item | Description |
|---|---|
| Product Name | Actinomycete Broth |
| Medium Type | Broth Culture Medium |
| Format | Powder |
| Package | 250 g / bottle |
| Storage | Room temperature |
| Shelf Life | 3 years |
| Application | Actinomycetes cultivation |