Acetamide Broth Medium – For Pseudomonas aeruginosa Ammonia Production Test, 250g

Acetamide Broth Medium (Acetamide Broth) is a selective biochemical medium used for the ammonia production test of Pseudomonas aeruginosa. This medium utilizes acetamide as the sole carbon and nitrogen source, promoting Pseudomonas metabolism while restricting the growth of most other bacteria.

The presence of ferrous sulfate and sodium molybdate allows the detection of ammonia production through metabolic activity, making it a valuable tool for microbial differentiation and biochemical identification.

Complete Formula (per liter):

• Monopotassium Phosphate (KH₂PO₄): 1.0 g

• Magnesium Sulfate (MgSO₄): 0.2 g

• Acetamide: 2.0 g

• Sodium Chloride (NaCl): 0.2 g

• Ferrous Sulfate (FeSO₄·7H₂O): 0.5 mg

• Sodium Molybdate (Na₂MoO₄·2H₂O): 5 mg

• Final pH: 7.1 ± 0.1 (25°C)

⚠ Note: Insoluble precipitates may form after sterilization. This is normal and does not affect performance.

Preparation & Instructions:

1. Weigh 3.4 g of Acetamide Broth powder.

2. Suspend in 1 liter distilled or deionized water.

3. Heat to boiling until fully dissolved.

4. Dispense into test tubes.

5. Sterilize at 121°C for 15 minutes.

6. Cool and use for ammonia production tests of Pseudomonas aeruginosa.

Packaging & Storage:

• 250 g per bottle

• Shelf life: 3 years

• Storage: Keep sealed, at room temperature, in a cool dry place