Acetamide Agar – Selective Medium for Pseudomonas aeruginosa Isolation, 250g

Acetamide Agar (Acetamide Medium) is a selective culture medium designed for the isolation and differentiation of Pseudomonas aeruginosa. This medium uses acetamide as the sole carbon and nitrogen source, combined with phenol red as a pH indicator, enabling the detection of metabolic activity of Pseudomonas species.

The selective formulation suppresses the growth of most non-Pseudomonas organisms, providing reliable results for microbiological research and diagnostic testing.

Complete Formula (per liter):

• Acetamide: 10.0 g

• Sodium Chloride (NaCl): 5.0 g

• Monopotassium Phosphate (KH₂PO₄, anhydrous): 0.73 g

• Dipotassium Phosphate (K₂HPO₄, anhydrous): 1.39 g

• Magnesium Sulfate (MgSO₄·7H₂O): 0.5 g

• Phenol Red: 0.012 g

• Agar: 20.0 g

• Final pH: 7.2 ± 0.1 (25°C)

Preparation & Instructions:

1. Weigh 37.6 g of Acetamide Agar powder.

2. Suspend in 1 liter distilled or deionized water.

3. Heat to boiling for 1 minute until fully dissolved.

4. Dispense into appropriate containers.

5. Sterilize at 121°C for 20 minutes.

6. Cool to solidify, then use for the isolation of Pseudomonas aeruginosa.

Packaging & Storage:

• 250 g per bottle

• Shelf life: 3 years

• Storage: Store sealed, in a cool and dry environment at room temperature