Acetamide Agar, 250g, selective culture medium for Pseudomonas aeruginosa differentiation and isolation

Acetamide Agar for Pseudomonas aeruginosa Isolation | Microbiology Culture Medium Powder 250g

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Acetamide Agar is a dehydrated microbiological culture medium used for the isolation and identification of Pseudomonas aeruginosa in laboratory microbiology testing. The medium is supplied as a powder and is prepared by dissolving in purified water followed by sterilization before use in microbiological analysis.

Product Overview

Acetamide Agar is a microbiological culture medium formulated for the isolation and cultivation of Pseudomonas aeruginosa in laboratory testing.

This dehydrated powder medium is widely used in microbiology laboratories for the detection and differentiation of Pseudomonas species in environmental, industrial, and research samples. The medium is prepared by dissolving the powder in purified water followed by sterilization and agar plate preparation.

The formulation supports microbiological testing procedures that require selective cultivation conditions for Pseudomonas aeruginosa.


Product Specifications

ParameterDescription
Product NameAcetamide Agar
ApplicationIsolation of Pseudomonas aeruginosa
Packaging250 g / bottle
StorageRoom temperature
Shelf Life3 years
FormDehydrated powder

Applications

Acetamide Agar is commonly used for:

  • Isolation of Pseudomonas aeruginosa
  • Microbiology laboratory testing
  • Environmental microbiology analysis
  • Water and industrial microbial monitoring
  • Research and quality control laboratories

Medium Composition (per liter)

Acetamide — 10.0 g
Sodium Chloride — 5.0 g
Monopotassium Phosphate — 0.73 g
Dipotassium Phosphate — 1.39 g
Magnesium Sulfate (7H₂O) — 0.5 g
Phenol Red — 0.012 g
Agar — 20.0 g

Final pH: 7.2 ± 0.1 (25°C)


Preparation Instructions

  1. Suspend 37.6 g of the medium in 1 liter of distilled or deionized water.
  2. Heat the mixture and boil for approximately 1 minute until the medium is completely dissolved.
  3. Dispense the medium into suitable containers.
  4. Sterilize by autoclaving at 121°C for 20 minutes.
  5. Allow the medium to cool before use.

Prepared medium can then be used for microbiological culture and isolation procedures.

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