7.5% Sodium Chloride Broth – Selective Enrichment for Staphylococcus aureus (250g)

7.5% Sodium Chloride Broth is a selective enrichment medium widely used for the isolation and homogenization of Staphylococcus aureus from food, clinical, and environmental samples. The high salt concentration (7.5% NaCl) inhibits most competing microorganisms while allowing the selective growth of staphylococci.

Key Applications:

• Enrichment of Staphylococcus aureus from food, cosmetics, and water samples

• Selective homogenization in microbiological testing

• Use in public health laboratories and food safety research

Composition (per liter):

• Peptone – 10.0 g

• Beef Extract – 5.0 g

• Sodium Chloride – 75.0 g

• Final pH: 7.4 ± 0.1 (at 25°C)

Preparation & Instructions:

1. Suspend 90.0 g of the medium in 1 liter of distilled or deionized water.

2. Warm gently to dissolve completely.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121°C for 15 minutes.

5. Allow to cool before use.

Packaging & Storage:

• 250 g per bottle

• Shelf life: 3 years

• Storage conditions: Room temperature, dry place, tightly closed