3% NaCl Alkaline Peptone Water – 250g | Vibrio parahaemolyticus Enrichment Broth

3% NaCl Alkaline Peptone Water is a selective enrichment medium optimized for primary isolation of Vibrio parahaemolyticus from seafood, marine environments, and clinical specimens, leveraging alkaline pH to inhibit competing flora.

Key Applications:

✔️ Pre-enrichment for V. parahaemolyticus per FDA BAM Chapter 9

✔️ Screening in seafood processing facilities (HACCP programs)

✔️ Clinical stool sample processing for vibrio infections

✔️ Environmental monitoring of marine waters

Targeted Formula (Per Liter):

| Component | Quantity |

|——————|———-|

| Peptone | 10.0 g |

| Sodium Chloride | 30.0 g |

Final pH: 8.5 ± 0.2 (25°C)

Critical Advantages:

– Dual selective action:

– 3% NaCl: Promotes halophilic vibrio growth

– Alkaline pH (8.5): Suppresses Gram-positive & enteric bacteria

– Rapid resuscitation: Recovers stressed/injured V. parahaemolyticus cells

– Ready-to-use powder: Ensures batch consistency

– 3-year shelf life: Ambient storage without performance loss

Protocol Simplified:

1️⃣ Dissolve 40.0g powder in 1L distilled water

2️⃣ Autoclave at 121°C for 15 minutes

3️⃣ Cool to 35-37°C before inoculating samples

Compatibility:

– Validated for use with TCBS agar subculture

– Compatible with ISO/TS 21872-1:2017 workflow