2216E Marine Broth – 250g | Oceanic Bacteria Liquid Culture
True Seawater Simulation for Marine Bacteriology
2216E Marine Broth (Zobell 2216) precisely replicates oceanic mineral composition, delivering optimal enrichment for fastidious saltwater bacteria in liquid culture systems. This ATCC/DSMZ-standardized formulation is indispensable for marine microbial research.
Critical Applications:
– Enrichment of oligotrophic marine bacteria
– Phytoplankton-associated microbe cultivation
– Deep-sea vent sample incubation
– Marine pathogen proliferation studies
– Biofilm formation analysis
Key Advantages:
✓ Authentic Salinity Profile – 19.45g/L NaCl + 8 essential minerals
✓ High-Yield Cultivation – Supports demanding marine strains
✓ Certified Formulation – Complies with ATCC Medium 2 & DSMZ 514
✓ Rapid Solubility – Dissolves in 10 minutes with stirring
Formula Composition (per liter):
– Peptone: 5.0g
– Yeast Extract: 1.0g
– Sodium Chloride: 19.45g
– Magnesium Chloride: 5.9g
– Magnesium Sulfate: 3.24g
– Calcium Chloride: 1.8g
– Potassium Chloride: 0.55g
– Ferric Citrate: 0.1g
– Sodium Bicarbonate: 0.16g
– Potassium Bromide: 0.08g
– Strontium Chloride: 34mg
– Boric Acid: 22mg
– Sodium Fluoride: 2.4mg
– Ammonium Salt: 1.6mg
– Disodium Phosphate: 8mg
– Sodium Silicate: 4mg
– Final pH: 7.6 ± 0.2 (25°C)
Preparation Protocol:
1. Suspend 37.4g in 1L distilled water
2. Boil with continuous agitation ≤10 min
3. Autoclave at 121°C for 15 minutes
4. Cool to 25°C before use
Note: Minor mineral precipitates may form but do not impact performance.
Quality Validation:
– Osmolality: 1000-1100 mOsm/kg
– Growth promotion tested with Vibrio alginolyticus
– Sterility certified
Store dry at 15-30°C. Shelf life: 24 months.
