1% Dextrose Broth Medium powder 250g used for bacterial enrichment and initial isolation in microbiology laboratories

1% Dextrose Broth for Bacterial Enrichment

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1% Dextrose Broth Medium is a general-purpose dehydrated culture medium formulated for the primary isolation and enrichment of bacteria.
With 1% dextrose content and balanced nutrient composition, this broth supports the early growth and recovery of microorganisms from various samples.

The medium is suitable for routine microbiological analysis, enrichment culture prior to plating, and general laboratory use. Its powdered format ensures easy preparation, consistent performance, and extended shelf life, making it a reliable choice for food, environmental, and industrial microbiology laboratories.


🔬 Applications

  • Primary isolation of bacteria

  • Enrichment culture prior to solid media plating

  • Routine microbiological cultivation

  • General laboratory bacterial growth studies


📦 Product Specifications

  • Product Name: 1% Dextrose Broth Medium

  • Form: Dehydrated powder

  • Package Size: 250 g / bottle

  • Storage Condition: Room temperature, dry place

  • Shelf Life: 3 years


🧾 Medium Composition (per liter)

  • Peptone – 10.0 g

  • Sodium Chloride – 5.0 g

  • Beef Extract – 3.0 g

  • Dextrose – 10.0 g

Final pH: 7.2 ± 0.2 (25 °C)


⚙️ Directions for Use

  1. Suspend 28.0 g of the medium in 1 liter of distilled or deionized water.

  2. Warm gently with stirring until completely dissolved.

  3. Dispense into suitable containers.

  4. Sterilize by autoclaving at 121 °C for 15 minutes
    or alternatively at 115 °C for 30 minutes.

  5. Cool to room temperature before use.

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