This 0.1% Peptone Water is a dehydrated powder formulation designed for use as a sample diluent in microbiological testing. It is widely used in food, pharmaceutical, cosmetic, and environmental laboratories for the preparation of sample homogenates and serial dilutions prior to inoculation onto culture media.
Peptone water at a low concentration (0.1%) provides a mild nitrogen source and helps to maintain the viability of microorganisms during the dilution process without promoting significant growth. This ensures that the microbial population in the sample remains stable and representative before being transferred to selective or general-purpose media for enumeration.
The medium is prepared by dissolving 1.0 g of the dehydrated powder in 1,000 mL of purified water. The resulting solution is clear and has a pH of 7.1 ± 0.2, which is compatible with a wide range of microbiological procedures. The product is supplied as a 250g bottle, which is sufficient to prepare up to 250 liters of working dilution solution.
Intended Use
- Preparation of sample dilutions for microbiological testing.
- Homogenization of food, pharmaceutical, and environmental samples.
- Serial dilution in colony counting procedures.
- General laboratory use as a diluent.
Composition (per Liter)
- Peptone: 1.0 g
- Final pH: 7.1 ± 0.2 at 25°C
Principle of the Medium
- Peptone: Provides a minimal source of nitrogen and nutrients to maintain microbial viability without promoting active growth during the dilution step.
Quality Control
According to the manufacturer’s sensitivity testing, the prepared medium is inoculated with quality control strains and incubated at 20–25°C with aerobic incubation for 45 minutes, to verify that the diluent supports the recovery and viability of target microorganisms without causing inhibition.
Storage and Shelf Life
- Format: 250 g/bottle, dehydrated powder.
- Shelf Life: 3 years from date of manufacture.
- Storage: Store in a dry, dark place at 2–8°C. Keep tightly closed to prevent moisture absorption.
Q: What is 0.1% Peptone Water used for?
A: It is used as a sample diluent in microbiological testing. It helps to maintain the viability of microorganisms during dilution, homogenization, and serial dilution procedures without promoting significant growth.
Q: Why is the peptone concentration only 0.1%?
A: A low peptone concentration (0.1%) provides minimal nutrients to keep microorganisms viable without allowing them to multiply during the dilution step. This ensures that the microbial count in the sample remains representative of the original sample.
Q: How do I prepare the medium?
A: Weigh 1.0 g of the powder, dissolve in 1,000 mL of purified water with heating, dispense into containers, and autoclave at 121°C for 15 minutes.
Q: What is the shelf life and storage condition?
A: The shelf life is 3 years from the date of manufacture when stored at 2–8°C in a dry, dark place. Keep the bottle tightly closed.
Q: Can I use this product for clinical testing?
A: This product is intended for laboratory and industrial microbiological testing. It is not intended for clinical diagnosis.
| Parameter | Specification |
|---|---|
| Product Form | Dehydrated powder |
| Package Size | 250 g/bottle |
| Peptone | 1.0 g/L (0.1%) |
| pH | 7.1 ± 0.2 (at 25°C) |
| Yield | 1 g powder per liter of diluent |
| Sterilization | 121°C for 15 minutes |
| Shelf Life | 3 years |
| Storage | 2–8°C, dry, protected from light |
| Intended Use | Sample dilution (in vitro) |
