Key Features
- Designed for actinomycetes cultivation and isolation
- Suitable for Streptomyces and related microorganisms
- Powder microbiological agar medium
- Ideal for environmental microorganism research
- 250 g laboratory packaging
- Stable formulation with 3-year shelf life
Applications
- Cultivation of actinomycetes
- Streptomyces isolation and culture
- Environmental microorganism studies
- Soil microbiology research
- Academic microbiology laboratories
Medium Composition
Composition (per liter)
Humic Acid — 1.0 g
Calcium Carbonate (CaCO3) — 0.02 g
Disodium Hydrogen Phosphate (Na2HPO4) — 0.5 g
Magnesium Sulfate Heptahydrate (MgSO4·7H2O) — 0.05 g
Potassium Chloride (KCl) — 1.7 g
Ferrous Sulfate Heptahydrate (FeSO4·7H2O) — 0.01 g
Vitamin B1 — 0.5 mg
Vitamin B2 — 0.5 mg
Vitamin B6 — 0.5 mg
Nicotinic Acid — 0.5 mg
Inositol — 0.5 mg
Pantothenic Acid — 0.5 mg
Biotin — 0.25 mg
p-Aminobenzoic Acid — 0.5 mg
Nalidixic Acid — 20.0 mg
Agar — 20.0 g
Final pH: 7.2 ± 0.2 (25°C)
Directions for Use
- Suspend 23.3 g of the medium in 1 liter of distilled or deionized water.
- Heat with stirring and boil for at least 1 minute until the agar is completely dissolved.
- Dispense into suitable containers.
- Sterilize by autoclaving at 121°C for 15 minutes.
- Allow the medium to cool before pouring into sterile petri dishes if agar plates are required.
Specification
| Item | Description |
|---|---|
| Product Name | Humic Acid HV Medium |
| Alternative Name | HV Agar Medium |
| Format | Powder |
| Package | 250 g / bottle |
| Storage | Room temperature |
| Shelf Life | 3 years |
| Application | Actinomycetes cultivation |