Medium 40 (250g) for microbiological antibiotic potency testing

Antibiotic Medium No. 40 – USP Medium for Microbiological Potency Testing

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Product Overview

Antibiotic Medium No. 40 is a dehydrated agar-based culture medium developed for the microbiological determination of antibiotic potency.
The formulation supports consistent microbial growth and diffusion-based assay systems commonly used in pharmaceutical quality control.

This medium is referenced to USP standards and supplied as a stable powder, ensuring batch-to-batch consistency, ease of preparation, and reliable assay performance.


Principle of the Method

The microbiological assay measures the biological activity of antibiotics by observing the growth response of suitable microorganisms in an agar medium.
The degree of inhibition or growth correlates with the potency of the antibiotic under controlled conditions.

Key Features

  • Dehydrated agar medium
  • Designed for microbiological antibiotic potency testing
  • Suitable for agar diffusion assay techniques
  • USP-referenced formulation
  • Consistent performance and reproducibility
  • Long shelf life and easy preparation

Applications

  • Antibiotic potency determination
  • Agar-based microbiological assays
  • Pharmaceutical quality control laboratories
  • Microbiology testing facilities

✅ Product Specifications

  • Product Name: Antibiotic Medium No. 40
  • Form: Dehydrated powder
  • Package Size: 250 g
  • Storage Condition: Room temperature
  • Shelf Life: 3 years
  • Reference Standard: USP
  • Final pH: 6.7 ± 0.2 (25°C)

✅ Composition (per liter)

ComponentQuantity
Yeast Extract20.0 g
Polypeptone5.0 g
Dextrose10.0 g
Monobasic Potassium Phosphate2.0 g
Polysorbate 800.1 g
Agar10.0 g

All ingredients are listed exactly as provided. No functional descriptions added.


✅ Preparation Instructions

  1. Suspend 47.1 g of the medium in 1,000 mL of distilled or purified water.
  2. Heat with frequent agitation until completely dissolved.
  3. Dispense into appropriate containers.
  4. Sterilize by autoclaving at 121°C for 15 minutes.
  5. Cool before use.

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