Antibiotic Medium No. 32 – USP Agar Medium for Microbiological Potency Testing
Product Overview
Antibiotic Medium No. 32 is a dehydrated agar culture medium formulated for the microbiological determination of antibiotic potency. The balanced formulation supports reliable microbial growth and consistent performance in agar-based antibiotic bioassay systems.
This medium is referenced to USP standards and supplied in powdered form, ensuring ease of preparation, batch-to-batch consistency, and extended shelf life. It is suitable for routine use in pharmaceutical quality control and microbiology laboratories.
Principle of the Method
The assay is based on the diffusion of antibiotics in an agar matrix, where microbial growth response is related to the concentration or biological activity of the antibiotic being tested.
Principle of the Method
The assay is based on the diffusion of antibiotics in an agar matrix, where microbial growth response is related to the concentration or biological activity of the antibiotic being tested.
Key Features
Dehydrated agar medium
Designed for antibiotic potency determination
Suitable for agar diffusion assays
USP-referenced formulation
Consistent gel strength and performance
Easy laboratory preparation
Applications
Microbiological antibiotic potency testing
Agar diffusion bioassays
Pharmaceutical and microbiology quality control
✅ Product Specifications
Product Name: Antibiotic Medium No. 32
Form: Dehydrated powder
Package Size: 250 g / bottle
Storage Condition: Room temperature
Shelf Life: 3 years
Reference Standard: USP
Final pH (25°C): 6.6 ± 0.1
✅ Composition (per liter)
Component
Quantity
Peptone
6.0 g
Pancreatic Digest of Casein
4.0 g
Yeast Extract
3.0 g
Beef Extract
1.5 g
Dextrose
1.0 g
Manganese Sulfate
0.5 g
Agar
15.0 g
All components are fully listed as provided. No functional descriptions added.
✅ Preparation Instructions
Suspend 31.0 g of the product in 1,000 mL of distilled or deionized water (preparation volume may be adjusted proportionally).
