Medium Composition
Composition per liter:
- Tryptone — 10.0 g
- Beef Extract — 5.0 g
- Yeast Extract — 1.0 g
- Sodium Pyruvate — 10.0 g
- Glycine — 12.0 g
- Lithium Chloride — 5.0 g
- Agar — 20.0 g
Preparation Instructions
- Suspend 6.3 g of the medium in 95 ml of distilled or deionized water.
- Heat to boiling until completely dissolved.
- Adjust volume to final level if required.
- Sterilize by autoclaving at 121°C for 15 minutes.
- Mix well immediately after sterilization to prevent agar settling.
- Cool to approximately 50°C.
- Aseptically add 1 vial of egg yolk tellurite enrichment.
- Mix gently and pour plates.
