Brain Heart Infusion Agar (BHI Agar)

Brain Heart Infusion Agar (BHI Agar) is a nutrient-rich, dehydrated solid culture medium designed for the cultivation of a wide variety of fastidious and non-fastidious bacteria.
The formulation includes balanced calf brain infusion and beef heart infusion, providing essential growth factors, nitrogen sources, and energy components required for reliable microbial growth.

With its high nutritional content and consistent performance, BHI Agar is widely used in microbiology laboratories for routine bacterial isolation, cultivation, and maintenance.
The dehydrated powder format ensures convenient preparation, stable quality, and extended shelf life.

🔬 Product Applications

• General bacterial cultivation

• Growth of fastidious microorganisms

• Routine microbiology laboratory use

• Clinical, food, pharmaceutical, and research testing

• Isolation and maintenance of bacterial cultures

📦 Product Specifications

• Product Name: Brain Heart Infusion Agar

• English Name: Brain Heart Infusion Agar

• Also Known As: BHI Agar

• Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature, dry place

• Shelf Life: 3 years

🧾 Medium Composition (per liter)

• Dehydrated Calf Brain Infusion – 4.0 g

• Dehydrated Beef Heart Infusion – 4.0 g

• Pancreatic Digest of Casein – 16.0 g

• Peptone – 5.0 g

• Dextrose – 2.0 g

• Sodium Chloride – 5.0 g

• Disodium Phosphate (Na₂HPO₄) – 2.5 g

• Agar – 15.0 g

Final pH: 7.4 ± 0.2 (25 °C)

⚙️ Directions for Use

1. Suspend 53.5 g of the medium in 1 liter of distilled or deionized water.

2. Heat with stirring and boil until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121 °C for 15 minutes.

5. Mix gently after sterilization and pour into sterile Petri dishes.

6. Allow to solidify before use.