Zobell Marine Broth 2216 (2216E Broth) | Marine Bacteria Culture Medium

Zobell Marine Broth 2216, also known as 2216E Marine Broth or ZMB Medium, is a nutritionally balanced liquid culture medium designed for the cultivation of marine bacteria.
It closely simulates the ionic composition of natural seawater and supports the growth of a wide range of aerobic marine microorganisms.

🔬 Product Applications

• Cultivation of marine bacteria

• Enrichment and maintenance of marine microbial strains

• Marine microbiology research

• Environmental and seawater microbial studies

• Aquaculture and marine biotechnology laboratories

📦 Product Specifications

• Product Name: Zobell Marine Broth 2216

• Alternative Names: 2216E Marine Broth, Marine Bacteria Broth, ZMB Medium

• Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature (dry, tightly sealed)

• Shelf Life: 3 years

🧾 Medium Composition (per liter)

• Peptone – 5.0 g

• Yeast Extract – 1.0 g

• Ferric Citrate – 0.1 g

• Sodium Chloride – 19.45 g

• Magnesium Chloride – 5.9 g

• Magnesium Sulfate – 3.24 g

• Calcium Chloride – 1.8 g

• Potassium Chloride – 0.55 g

• Sodium Bicarbonate – 0.16 g

• Potassium Bromide – 0.08 g

• Strontium Chloride – 34.0 mg

• Boric Acid – 22.0 mg

• Sodium Silicate – 4.0 mg

• Sodium Fluoride – 2.4 mg

• Ammonium Salt – 1.6 mg

• Disodium Phosphate – 8 mg

Final pH: 7.6 ± 0.2 (25 °C)

⚙️ Directions for Use

1. Suspend 37.4 g of the medium in 1 liter of distilled or deionized water.

2. Heat to boiling with stirring until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121 °C for 15 minutes.

5. Cool to room temperature before use.