M-PA-C Agar (PA-C Agar, mPA-C Agar) – Selective Medium for Pseudomonas aeruginosa in Water (250g)

M-PA-C Agar (also known as PA-C Agar or mPA-C Agar) is a selective culture medium specifically formulated for the isolation, cultivation, and enumeration of Pseudomonas aeruginosa from water samples.

This medium is enriched with multiple carbohydrates (xylose, sucrose, lactose) for differentiation, while phenol red serves as a pH indicator to monitor fermentation reactions. Kanamycin and Nalidixic Acid are incorporated as selective agents to inhibit non-target organisms, ensuring the reliable recovery of Pseudomonas aeruginosa.

M-PA-C Agar is widely applied in:

• Water quality testing

• Environmental monitoring

• Microbiological research and diagnostics

⚠️ Important Note: This medium should not be autoclaved, as high temperatures may destroy its selective properties.

Composition (per liter):

• Yeast Extract – 2.0 g

• L-Lysine HCl – 5.0 g

• Sodium Chloride – 5.0 g

• Xylose – 1.25 g

• Sucrose – 1.25 g

• Lactose – 1.25 g

• Phenol Red – 0.08 g

• Ferric Ammonium Citrate – 0.8 g

• Sodium Thiosulfate – 5.0 g

• Magnesium Sulfate – 1.5 g

• Kanamycin – 8.0 mg

• Nalidixic Acid – 37.0 mg

• Agar – 12.0 g

• Final pH: 7.2 ± 0.1 (at 25°C)

Preparation & Instructions:

1. Suspend 35.0 g of medium in 1 L distilled or deionized water.

2. Heat to boiling for at least 1 minute until completely dissolved.

3. Do not autoclave.

4. Pour into sterile Petri dishes and allow to solidify.

5. Use immediately or store plates at 2–8°C for short-term use.

💡 Due to the high magnesium sulfate content, insoluble crystalline particles may appear after boiling. This is a normal phenomenon. Shake gently before pouring plates to ensure uniform distribution.