Raffinose-Histidine Medium – Agar-Based Medium for Actinomycete Isolation, 100g

Raffinose-Histidine Medium is a selective agar-based medium designed for the isolation and cultivation of Actinomycetes. The combination of raffinose as a carbon source and histidine as a nitrogen source provides an enriched environment that supports actinomycete differentiation while inhibiting unwanted microbial growth.

Complete Formula (per liter):

• Histidine: 0.5 g

• Raffinose: 2.5 g

• Potassium Phosphate Tribasic (K₂HPO₄·3H₂O): 1.0 g

• Magnesium Sulfate Heptahydrate (MgSO₄·7H₂O): 0.5 g

• Ferrous Sulfate Heptahydrate (FeSO₄·7H₂O): 0.01 g

• Calcium Chloride (CaCl₂): 0.02 g

• Agar: 20.0 g

• Final pH: 7.2 ± 0.2 at 25°C

Instructions for Use:

1. Dissolve 24.5 g of medium in 1 liter of distilled or deionized water.

2. Heat to boiling for at least 1 minute until the agar is completely dissolved.

3. Dispense into suitable containers.

4. Sterilize at 121°C for 15 minutes.

5. Cool and store until use.

Packaging & Storage:

• 100 g per bottle

• Shelf life: 3 years

• Storage: Store in a cool, dry place at room temperature