CFAT Agar Base for Actinomyces Isolation and Cultivation – 250g

CFAT Agar Base (Cadmium Sulfate Fluoride Acridine Trypticase Agar Base; also known as Cadmium Fluoride Acriflavin Tellurite Agar Base) is a specialized microbiological medium used for the selective isolation and cultivation of Nocardia and Actinomyces species. It is widely applied in microbial research for accurate identification of filamentous bacteria.

Medium Information:

• Form: Dehydrated culture medium powder

• Packaging: 250g per bottle

• Shelf Life: 3 years (store at room temperature)

Preparation Instructions:

1. Dissolve 50.0g of the medium in 980ml of distilled or deionized water.

2. Add:

   • Acriflavin solution (0.12mg) – 10 vials

   • Alkaline fuchsin solution – 10 vials

3. Heat to boiling for at least 1 minute until completely dissolved.

4. Dispense as required and sterilize by autoclaving at 121°C for 15 minutes.

5. After sterilization, shake gently, pour into plates, and allow to solidify.

6. Optionally, supplement with 5% sterile defibrinated sheep blood for specific applications.

This medium provides a selective environment suitable for Nocardia and Actinomyces, ensuring reliable microbial growth for laboratory studies.