Glycerol Glycine Agar for Streptomyces Cultivation and Identification – 250g

Glycerol Glycine Agar (Okazaki and Okami, 1976) is a specialized microbiological medium designed for the cultivation and identification of Streptomyces species. It provides essential nutrients to support optimal growth conditions for Streptomyces and other related actinobacteria. This medium is ideal for laboratory research and microbiological studies.

Each liter of the medium contains the following components:

• Glycine: 2.5g

• Sodium Chloride (NaCl): 1.0g

• Di-potassium Hydrogen Phosphate (K2HPO4): 1.0g

• Ferrous Sulphate (FeSO4): 0.1g

• Magnesium Sulphate (MgSO4): 0.1g

• Calcium Carbonate (CaCO3): 0.1g

• Agar: 15.0g

The pH is adjusted to 7.0 ± 0.2 at 25°C, making it suitable for the growth of Streptomyces species.

Instructions for Use:
To prepare the medium, dissolve 19.8g of the agar in 1L of deionized water containing 50% seawater. Add 20ml of glycerol, and adjust the pH to 7.4 using NaOH or dilute HCl. Boil the mixture for at least one minute to ensure complete dissolution. Sterilize the medium by autoclaving at 121°C for 15 minutes. After sterilization, the medium is ready for use.

Storage:
Store at room temperature (25°C). The shelf life of the product is 3 years.