Modified Starkey’s Medium C with 2.5% NaCl Starkey’s Medium Base, Modified with 2.5% NaCl ATCC Medium 210 / ATCC Medium: 210 Starkey’s Medium C, Modified with Salt
The Modified Starkey’s Medium C with 2.5% NaCl is designed for the growth of Desulfobacter and Desulfovibrio species, as well as other sulfate-reducing bacteria. The addition of 2.5% NaCl enhances its utility for more specific environments and is optimized for sulfur reduction studies. It includes 35% Sodium Lactate Solution to further stimulate growth and ensure the desired microbial activity.
Formulation (Per Liter of Medium):
NaCl (Sodium Chloride): 25.0g
Yeast Extract: 1.0g
Potassium Dihydrogen Phosphate (KH₂PO₄): 0.5g
Ammonium Chloride (NH₄Cl): 1.0g
Sodium Sulfate (Na₂SO₄): 1.0g
Calcium Chloride Dihydrate (CaCl₂·2H₂O): 0.1g
Magnesium Sulfate Heptahydrate (MgSO₄·7H₂O): 2.0g
L-Cysteine Hydrochloride (L-Cysteine·HCl): 0.75g
Ferrous Ammonium Sulfate (Fe(NH₄)₂(SO₄)₂): 0.5g
Usage Instructions:
Dissolve 31.85g of Modified Starkey’s Medium C with 2.5% NaCl in 1L of distilled or deionized water.
Heat and stir the solution until fully dissolved.
Sterilize by autoclaving at 121°C for 15 minutes.
After sterilization, cool the medium to room temperature (25°C).
Add 1 vial of 35% Sodium Lactate Solution (contains 0.35g sodium lactate per vial) to every 100ml of the medium.
Adjust the pH to 7.4 at 25°C using sterile Sodium Carbonate Solution or diluted Hydrochloric Acid.
Mix well, and the medium is ready for use.
