MacConkey Agar No.2 for Selective Isolation of Enteric Pathogens – 250g

MacConkey Agar No.2 is a differential and selective medium used for the isolation and cultivation of enteric pathogens, particularly those from the Enterobacteriaceae family. This agar is specifically formulated to support the growth of Gram-negative bacteria while inhibiting Gram-positive organisms. It is commonly used in microbiological laboratories for testing food, water, and clinical samples for pathogenic enteric bacteria.

Key Features:

• Selective and differential medium for isolating enteric pathogens.

• Contains bile salts to inhibit Gram-positive organisms and neutral red for lactose fermentation detection.

• High-quality ingredients ensure reliable and accurate results.

• Sterilization ready: Easily prepared for use by autoclaving.

• Long shelf life: Stable for up to 3 years at room temperature.

Instructions for Use:
To prepare, dissolve 51.5g of MacConkey Agar No.2 in 1L of distilled or deionized water. Boil for 1 minute to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes, then cool to 55°C and pour into petri dishes. Allow to solidify before use.

Storage: Store at room temperature, away from direct sunlight. Shelf life: 3 years.

Ingredients per liter:

• Peptone: 20.0g

• Lactose: 10.0g

• Bile Salt No.2: 1.5g

• Sodium Chloride (NaCl): 5.0g

• Neutral Red: 0.05g

• Crystal Violet: 0.001g

• Agar: 15.0g

• pH: 7.2 ± 0.2 at 25°C