SBG Sulfa Enrichment Broth – Modified Selenite Brilliant Green Broth for Salmonella, 250g

SBG Sulfa Enrichment Broth (Modified Selenite Brilliant Green Broth, SBG) is a selective enrichment medium containing sulfapyridine, specifically designed for selective enrichment of Salmonella species from clinical, food, and environmental samples.

The medium combines sodium selenite, brilliant green, and sulfapyridine to inhibit competing flora while promoting the growth of Salmonella. Mannitol serves as a fermentable carbohydrate to support bacterial metabolism.

Product Information

• Product Name: SBG Sulfa Enrichment

• English Name: Selenite Brilliant Green Broth, Modified

• Pack Size: 250g per bottle

• Form: Dehydrated culture medium powder

• Storage: Room temperature

• Shelf Life: 3 years

Applications

• Selective enrichment of Salmonella spp.

• Microbiological testing in food, clinical, and environmental samples

• Suitable for laboratories requiring highly selective enrichment media

Composition (per liter)

• Yeast Extract: 5.0 g

• Peptone: 5.0 g

• D-Mannitol: 5.0 g

• Sodium Taurocholate: 1.0 g

• Sodium Sulfapyridine: 0.5 g

• Sodium Selenite: 4.0 g

• Dipotassium Phosphate: 2.65 g

• Monopotassium Phosphate: 1.02 g

• Brilliant Green: 5.0 mg

• Final pH: 7.2 ± 0.2 at 25℃

Preparation Instructions

1. Dissolve 24.2 g of dehydrated medium in 1 liter of distilled or deionized water.

2. Heat gently for 5–10 minutes to dissolve completely. Avoid excessive heating and do not autoclave, as this may affect the selective properties.

3. Dispense into sterile containers under aseptic conditions.

Safety Notes

• For laboratory use only.

• Handle pathogenic organisms under appropriate biosafety conditions.