Martin-Lewis Broth Base – 250g for Selective Enrichment of Pathogenic Neisseria

Martin-Lewis Broth Base is a selective enrichment medium designed for the growth of pathogenic Neisseria species, particularly Neisseria gonorrhoeae. This medium promotes selective proliferation of gonococci while suppressing contaminating bacteria. It is used in clinical diagnostics and laboratory research.

Product Information

• Product Name: Martin-Lewis Broth Base

• Pack Size: 250g per bottle

• Form: Dehydrated powder

• Storage: Room temperature

• Shelf Life: 3 years

Applications

• Selective enrichment culture of pathogenic Neisseria

• Laboratory research and clinical diagnostics

• Supports growth with IsoVitaleX additive and VCNT inhibitor

Preparation Instructions

1. Dissolve 2.6 g of the medium in 50 ml of distilled or deionized water.

2. Autoclave at 121℃ for 15 minutes and cool to room temperature.

3. Aseptically dissolve 1 g sterile hemoglobin powder in 50 ml sterile water (add glass beads before sterilization for better dissolution) and preheat to 50℃.

4. Combine hemoglobin solution with the cooled broth base.

5. Add 1 vial of IsoVitaleX additive and 1 vial of VCNT selective inhibitor per 50 ml medium, mix gently.

6. Ready for selective enrichment culture of Neisseria gonorrhoeae.

Notes

• Laboratory use only.

• Handle pathogenic organisms under appropriate biosafety conditions.