Rogosa SL Broth – Selective Medium for Lactobacillus Detection, 250g

Rogosa SL Broth is a selective culture medium widely used for the detection and cultivation of Lactobacillus species in oral, vaginal, and fecal samples. Its carefully balanced composition with multiple carbon sources and selective inhibitors makes it highly effective for microbiological testing and probiotic quality control.

Product Information

• Product Name: Rogosa SL Broth

• Form: Dehydrated powder

• Pack Size: 250g per bottle

• Storage: Room temperature

• Shelf Life: 3 years

Medium Composition (per liter)

• Tryptone – 10.0 g

• Yeast Extract – 5.0 g

• Dextrose – 10.0 g

• Arabinose – 5.0 g

• Sucrose – 5.0 g

• Sodium Acetate – 15.0 g

• Ammonium Citrate – 2.0 g

• Monopotassium Phosphate – 6.0 g

• Magnesium Sulfate – 0.57 g

• Manganese Sulfate – 0.12 g

• Ferrous Sulfate – 0.03 g

• Final pH: 5.4 ± 0.2 at 25℃

Preparation Instructions

1. Suspend 58.7 g of the medium in 1 liter of distilled or deionized water.

2. Heat to boiling until completely dissolved.

3. Add 1 g Tween 80 and 1.32 ml glacial acetic acid, mix thoroughly.

4. Adjust pH to 5.4 ± 0.2 (at 25℃).

5. Boil for 2–3 minutes. Do not autoclave.

6. Cool to room temperature before use.

Applications

• Selective cultivation and detection of Lactobacillus spp.

• Testing in oral, vaginal, and fecal microbiota research

• Probiotic quality control and clinical microbiology studies