LAPT Broth – Nutrient Medium for Lactic Acid Bacteria Cultivation

LAPT Broth is a nutrient-rich medium designed for the cultivation of lactic acid bacteria (LAB). It contains peptone, tryptone, yeast extract, and Tween 80, providing essential nutrients for robust bacterial growth.

Product Information:

• Name: LAPT Broth

• Form: Dehydrated powder

• Pack Size: 250g per bottle

• Storage: Room temperature

• Shelf Life: 3 years

Composition (per liter):

• Peptone – 15.0 g

• Tryptone – 10.0 g

• Yeast Extract – 10.0 g

• Tween 80 – 1.0 g

• pH 6.5 ± 0.2 (25℃)

Preparation Instructions:

1. Dissolve 36.0 g of powder in 1 L of distilled or deionized water.

2. Optional: Add selective carbon sources such as glucose, fructose, galactose, lactose, or sucrose as required.

3. Adjust pH to 6.5 ± 0.2 after supplementation if needed.

4. Dispense and sterilize at 118℃ for 20 minutes.

5. Cool and use as needed for LAB cultivation.

Notes:

• Ideal for enhanced growth of lactic acid bacteria.

• Contains Tween 80 to facilitate bacterial dispersion.