Plate Count Agar with BCP – For Total Lactic Acid Bacteria Count

Plate Count Agar with BCP is a specialized agar medium formulated for enumeration of total lactic acid bacteria (LAB). The addition of Brom Cresol Purple (BCP) allows clear visualization of microbial growth, facilitating accurate colony counting in fermented products.

Product Information:

• Name: Plate Count Agar with BCP

• Form: Dehydrated powder

• Pack Size: 250g per bottle

• Storage: Room temperature

• Shelf Life: 3 years

Composition (per liter):

• Peptone – 5.0 g

• Yeast Extract – 2.5 g

• Dextrose – 1.0 g

• Polysorbate 80 (Tween 80) – 1.0 g

• L-Cysteine – 0.1 g

• Brom Cresol Purple (BCP) – 0.06 g

• Agar – 15.0 g

• pH: 7.0 ± 0.2 (25℃)

Preparation Instructions:

1. Dissolve 24.7 g of powder in 1 L of distilled or deionized water.

2. Heat to boiling until fully dissolved.

3. Dispense and sterilize by autoclaving at 121℃ for 15 minutes.

4. After sterilization, mix thoroughly to prevent agar settling.

5. Pour into plates and allow to solidify. Medium is ready for total LAB counting.

Applications:

• Enumeration of total lactic acid bacteria in fermented products

• Microbiological quality control of dairy, fermented foods, and beverages

• Colony counting with clear differentiation using Brom Cresol Purple