APT Agar – Medium for Selective Isolation and Cultivation of Lactic Acid Bacteria

APT Agar is a specialized medium formulated for the selective isolation and cultivation of lactic acid bacteria (LAB). Widely used in food, dairy, and microbiology laboratories, this medium provides optimal nutrients and minerals to support LAB growth.

Product Information:

• Name: APT Agar

• Form: Dehydrated powder

• Pack Size: 250g per bottle

• Storage: Room temperature

• Shelf Life: 3 years

Composition (per liter):

• Yeast Extract – 7.5 g

• Pancreatic Digest of Casein – 12.5 g

• Dextrose – 10.0 g

• Sodium Chloride – 5.0 g

• Sodium Citrate – 5.0 g

• Thiamine•HCl – 0.01 g

• Dipotassium Hydrogen Phosphate – 5.0 g

• Manganese Chloride – 0.14 g

• Magnesium Sulfate – 0.8 g

• Ferrous Sulfate – 0.06 g (0.02 g + 0.04 g)

• Tween 80 – 0.2 g

• Agar – 15.0 g

• pH: 6.7 ± 0.2 (25℃)

Preparation Instructions:

1. Dissolve 61.2 g of APT Agar powder in 1 L of distilled or deionized water.

2. Heat and boil until fully dissolved.

3. Dispense into containers and sterilize by autoclaving at 121℃ for 15 minutes.

4. Cool and gently shake to prevent agar from settling at the bottom.

5. Ready for the selective cultivation of lactic acid bacteria.