Trypticase Soy Polymyxin Broth Base – TSPB Medium 250g

Trypticase Soy Polymyxin Broth Base (TSPB) is a microbiological medium used for the Most Probable Number (MPN) test of Bacillus cereus. This broth base provides a reliable formulation for bacterial detection and enumeration in laboratory and research applications.

Product Details:

• Name: Trypticase Soy Polymyxin Broth Base (TSPB)

• Pack Size: 250g per bottle

• Storage: Room temperature

• Shelf Life: 3 years

• pH: 7.3 ± 0.1 at 25°C

Medium Composition (per liter):

• Pancreatic Digest of Casein 17.0g

• Enzymatic Digest of Soybean Meal 3.0g

• Sodium Chloride 5.0g

• Dextrose 2.5g

• Dipotassium Hydrogen Phosphate 2.5g

Preparation Instructions:

1. Dissolve 30.0g of the medium in 1L of distilled or deionized water with gentle warming.

2. Dispense 15ml into each test tube.

3. Sterilize by autoclaving at 121°C for 15 minutes.

4. After cooling below 50°C, aseptically add 0.3ml of S1302 per tube before use.

5. Alternatively, after sterilization, add one vial of TSPB supplement per 100ml medium, mix well, and dispense into sterile tubes.

This Trypticase Soy Polymyxin Broth Base ensures consistent performance for Bacillus cereus MPN testing, making it suitable for microbiological research, quality control, and food safety analysis.