
M-Endo Broth Medium for Coliform Detection in Water (250g)
M-Endo Broth Medium is a selective liquid culture medium formulated for the detection of coliform bacteria in water samples using the membrane filtration method. It is widely applied in microbiological water testing laboratories for reliable and consistent results.
Product Name: M-Endo Broth Medium
Form: Dehydrated liquid culture medium
Package Size: 250g per bottle
Storage Condition: Store at room temperature
Shelf Life: 3 years
Composition (per liter)
Pancreatic Digest of Casein – 10.0 g
Peptone – 10.0 g
Yeast Extract – 1.5 g
Lactose – 12.5 g
Sodium Chloride – 5.0 g
Dipotassium Phosphate – 4.375 g
Monopotassium Phosphate – 1.375 g
Sodium Lauryl Sulfate – 0.05 g
Sodium Desoxycholate – 0.1 g
Sodium Sulfite – 2.1 g
Basic Fuchsin – 1.05 g
pH: 7.2 ± 0.1 (25°C)
Preparation Instructions
Weigh 4.8g of the medium and dissolve in 98ml of distilled or deionized water. Add 2ml of absolute ethanol. Heat the mixture until boiling, then immediately stop heating. Cool to below 45°C. Store at 2–10°C and use within 96 hours.