Pfizer Enterococcus Selective Agar (PSE Agar) – Bile-Enriched Medium for Enterococci, 250g

Pfizer Enterococcus Selective Agar (PSE Agar) is formulated for the selective isolation and cultivation of fecal streptococci (enterococci). Its composition with bile powder, peptone, yeast extract, esculin, ferric citrate, sodium citrate, and selective inhibitors ensures high selectivity while supporting enterococci growth. Widely used in food, water, and clinical microbiology laboratories, it is ideal for detecting and enumerating enterococci.

Composition (per liter):

• Peptone – 20.0 g

• Yeast Extract – 5.0 g

• Bile Powder – 10.0 g

• Sodium Chloride – 5.0 g

• Sodium Citrate – 1.0 g

• Esculin – 1.0 g

• Ammonium Ferric Citrate – 0.5 g

• Inhibitor – 0.25 g

• Agar – 15.0 g

• Final pH: 7.1 ± 0.2 (at 25℃)

Preparation Instructions:

1. Dissolve 57.8 g of dehydrated medium in 1 liter of distilled or deionized water.

2. Heat to boiling with stirring until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121℃ for 15 minutes or 115℃ for 30 minutes.

5. Mix immediately after sterilization to prevent agar sedimentation and solidification at the bottom.

6. Cool to 50–55℃ and store under aseptic conditions until use.

Storage:
Store in a tightly sealed container at room temperature in a dry environment. Shelf life: 3 years.

Applications:

• Selective isolation of enterococci (fecal streptococci)

• Food safety testing and microbiological water analysis

• Clinical and laboratory use for bacterial enumeration