Christensen Citrate Agar for Bacterial Utilization Testing
Standardized Slant Medium for Citrate Assimilation Assays
This Christensen-formulated citrate agar detects bacterial utilization of citrate as sole carbon source through distinctive color transition. Prussian blue indicator provides visual alkaline reaction confirmation.
Key Diagnostic Features:
Positive result: Deep blue color development
Negative result: Original green coloration
Gas production: Visible fissures in slant
Technical Specifications:
▶ Preparation Protocol:
Prepare per manufacturer’s instructions
Solidify as slants with 1.5-2cm butt
Sterilize at 115°C for 20min
▶ Incubation: 18-24h at 35±2°C
Critical Applications:
IMViC test component for Enterobacteriaceae
Differentiation of Salmonella vs. Shigella
Environmental microbe screening
