Test tube with semisolid agar displaying yellow acid production and gas fissures

Glucose Semisolid Agar for Acid/Gas Detection

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Dual-Purpose Medium for Glucose Fermentation & Motility Testing
This semisolid agar with bromcresol purple indicator simultaneously detects acid production (color change) and gas formation (fissures) in bacterial glucose metabolism studies.

Key Features:

  • Low agar concentration (3g/L) for optimal motility observation

  • Pre-mixed glucose (10g/L) for immediate fermentation

  • Room temperature stable (3-year shelf life)

  • Clear purple-to-yellow transition at pH shift

Technical Specifications:
▶ Formula (per liter):
Peptone 10.0g | Beef Extract 3.0g | NaCl 5.0g
Dextrose 10.0g | BCP 0.016g | Agar 3.0g
▶ pH: 7.4±0.2 (25°C)
▶ Preparation: 31g/L → boil → sterilize at 121°C/15min → solidify upright

Diagnostic Applications:

  • Differentiation of Enterobacteriaceae

  • Detection of non-fermenting bacteria

  • Microbial motility characterization

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