Lead Acetate Test Base for Bacterial H2S Detection

Specialized Medium for Hydrogen Sulfide Production Testing
This base medium requires addition of lead acetate solution to detect black precipitate formation in bacterial H2S tests. Essential for differentiating Salmonella, Citrobacter, and other sulfide-producing pathogens.

⚠️ Critical Safety Note:
△ Must add sterile 10% lead acetate post-sterilization
△ For trained microbiologists only

Key Features:

• Pre-measured sodium thiosulfate (sulfur source)

• Optimized agar concentration for clear slant formation

• Room temperature stable (3-year shelf life)

• Complies with standard H2S test protocols

Technical Specifications:
▶ Formula (per liter equivalent):
Peptone 10.0g | Beef Extract 3.0g | NaCl 5.0g
Na₂S₂O₃ 2.5g | Agar 15.0g
▶ pH: 7.3±0.1 (25°C)
▶ Preparation:

1. 35.5g/L → boil → 121°C/15min sterilization

2. Cool 50-55°C → add 10ml 10% sterile lead acetate

Diagnostic Applications:

• Enterobacteriaceae identification

• Food/water safety testing

• Clinical isolate characterization