Sealed bottle of ITC Broth Base powder with additive vials - 250g for selective Yersinia culture

Selective ITC Enrichment Broth | Yersinia Culture | Additive Required | 250g

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ITC Selective Broth Base for Enhanced Yersinia Recovery
Specialized enrichment medium optimized for Yersinia species isolation. Requires ITC Supplement additive (1 vial per 100mL) added post-sterilization to suppress competing flora. High-magnesium formulation maintains critical osmotic balance for pathogen growth.

Critical Specifications:

  • Application: Selective enrichment of Yersinia spp.

  • Essential Additive: ITC Supplement (sold separately)

  • Format: Dehydrated powder

  • Preparation: 44.0g/L distilled water

  • Sterilization: 121°C for 15 minutes

  • Additive Integration: At 47±2°C

  • pH: 6.9±0.2 (25°C)

  • Packaging: 250g/bottle

  • Shelf Life: 3 years at room temperature

Protocol:

  1. Dissolve 44.0g in warm water (40-50°C)

  2. Sterilize and cool to 47°C

  3. Aseptically add 1 ITC Supplement vial per 100mL

  4. Mix before use

Note: Malachite green content requires standard laboratory safety practices.

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