APM medium plates showing pyrazinamidase test results for Aeromonas

APM Medium – 100g | Pyrazinamidase Activity Test for Aeromonas Identification

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APM Medium (Aeromonas Pyrazinamidase Medium) is designed for detecting pyrazinamidase enzyme activity in Aeromonas species, a critical biochemical test for species-level identification.

Key Application:

✔️ Differentiation of A. hydrophila from A. caviae

✔️ Pyrazinamidase activity confirmation

✔️ Clinical and environmental strain identification

Unique Preparation:

– Requires 0.2M Tris-maleate buffer (not water)

– Dissolve 34.0g in 1L buffer

Test Principle:

– Pyrazinamidase-positive strains: Color change

A. hydrophila: Typically positive

A. caviae: Typically negative

Protocol:

1️⃣ Prepare 0.2M Tris-maleate buffer

2️⃣ Suspend 34.0g in 1L buffer

3️⃣ Boil 1 minute

4️⃣ Autoclave at 121°C for 15 minutes

Features:

– Ready-to-use powder

– 100g convenient size

– 3-year ambient stability

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