Casein Soybean Digest Broth – 250g | Microbial Culture Media for Sterility Testing

Casein Soybean Digest Broth is a general-purpose culture medium designed for reliable bacterial growth in pharmaceutical quality control, environmental monitoring, and diagnostic applications.

Key Applications:

✔️ Sterility testing of medical devices & parenteral products

✔️ Bioburden testing according to USP <61> and EP 2.6.12

✔️ Enrichment culture for fastidious microorganisms

✔️ Growth promotion testing of culture media

Optimized Formula Per Liter:

| Component | Quantity |

|————————————|———-|

| Pancreatic Digest of Casein | 17.0 g |

| Enzymatic Digest of Soybean Meal | 3.0 g |

| Sodium Chloride | 5.0 g |

| Dextrose | 2.5 g |

| Dipotassium Hydrogen Phosphate | 2.5 g |

Final pH: 7.3 ± 0.2 (25°C)

Simplified Protocol:

1️⃣ Dissolve 30.0g powder in 1L distilled water

2️⃣ Sterilize by autoclaving at 121°C for 15 minutes

3️⃣ Cool to 45-50°C before use

Product Advantages:

– Dual enzymatic digests: Pancreatic casein + Papain soybean for balanced nutrient profile

– Buffered system: K₂HPO₄ maintains pH stability during growth

– Ready-to-use powder: Reduces preparation errors

– 3-year shelf life: Ambient storage without viability loss

Quality Assurance:

– Batch-to-batch consistency

– Low particulate content for clear broth observation