Bacillus atrophaeus Agar Medium – 250g | Ready-to-Use Culture Media for Spore Testing

Precision-formulated Bacillus atrophaeus Agar Medium for standardized cultivation of spore-forming bacteria in sterility testing, environmental monitoring, and pharmaceutical quality control.

Key Applications:

✔️ Growth and maintenance of Bacillus atrophaeus (formerly B. subtilis black variant)

✔️ Preparation of spore suspensions for disinfectant efficacy testing

✔️ Microbial limit testing (MLT) in compliance with USP/EP standards

Optimized Formula Per Liter:

| Component | Quantity |

|——————–|———-|

| Peptone | 10.0 g |

| Beef Extract | 3.0 g |

| Sodium Chloride | 5.0 g |

| Manganese Sulfate | 0.005 g |

| Agar | 15.0 g |

Final pH: 7.3 ± 0.2 (25°C)

Protocol Simplified:

1️⃣ Dissolve 33.0g powder in 1L distilled water

2️⃣ Autoclave at 121°C for 15 minutes

3️⃣ Mix gently post-sterilization to prevent agar settling

Quality Assurance:

– Consistent spore formation support

– Batch-to-batch reproducibility

– 3-year shelf life at room temperature

Order Convenience:

– 250g/bottle ready-to-use powder

– Ships globally in temperature-stable packaging