Buffered Peptone Saline Solution pH 7.0 – Universal Sample Diluent (250g)
Pharmacopoeia-Compliant Buffered Diluent for Microbial Sample Preparation
This precision-formulated solution combines phosphate-buffered saline (0.067mol/L phosphate) with 1.0g/L peptone to maintain osmotic balance and microbial viability during sample dilution. With strict pH control at 7.0±0.1, it meets international pharmacopoeial requirements for sample preparation in pharmaceutical, food, and environmental testing.
Key Applications:
🧪 Sample Dilution: Ideal for serial dilution of microbial suspensions
💊 Pharmaceutical Testing: Complies with pharmacopoeial sample preparation protocols
🥫 Food Microbiology: Preserves cell viability in food homogenates
🌡️ Environmental Monitoring: Diluent for water and surface samples
Technical Advantages:
✅ Dual Buffer System: 3.6g/L KH₂PO₄ + 7.2g/L Na₂HPO₄ (0.067M phosphate)
✅ Osmotic Protection: 4.3g/L NaCl maintains cell integrity
✅ Nutrient Base: 1.0g/L peptone prevents cell shock
✅ pH Precision: Maintains 7.0±0.1 during dilution procedures
✅ Sterilization Flexibility: Validated for 121°C/15min or 115°C/30min
Preparation Protocol:
Suspend 16.1g in 1L purified water
Warm to 45-50°C for complete dissolution
Dispense into appropriate containers
Autoclave using preferred protocol
Complete Formulation (Per Liter):
Peptone: 1.0g
KH₂PO₄: 3.6g
Na₂HPO₄·2H₂O: 7.2g
NaCl: 4.3g
