Sealed bottle of BPY Agar powder

BPY Agar – Nutrient-Rich Medium with Beef, Peptone & Yeast Extract (250g)

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Enhanced BPY Agar for Demanding Bacterial Cultures
This enriched formulation combines beef extract (5.0g/L), peptone (10.0g/L) and yeast extract (5.0g/L) with 5.0g/L glucose to support robust growth of nutritionally demanding bacteria. The pH-stabilized (7.0±0.2) medium is particularly effective for fastidious organisms and industrial strain maintenance.

Key Applications:

  • 🧫 Fastidious Bacteria: Ideal for streptococci and other challenging species

  • 🏭 Culture Preservation: Maintains viability for stock cultures

  • 🔬 Research Studies: Supports consistent experimental results

Technical Advantages:

  • Triple Nutrient Base: Beef/peptone/yeast extract combination

  • Energy-Rich: 5.0g/L glucose promotes rapid growth

  • Optimal Solidification: 15.0g/L agar for perfect plates

  • Standardized: 45.0g/L preparation concentration

Preparation Protocol:

  1. Suspend 45.0g in 1L purified water

  2. Boil until complete dissolution

  3. Autoclave at 121°C for 15 minutes

  4. Pour plates at ≈50°C

Complete Formulation (Per Liter):

  • Beef Extract: 5.0g

  • Peptone: 10.0g

  • Yeast Extract: 5.0g

  • Glucose: 5.0g

  • NaCl: 5.0g

  • Agar: 15.0g

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