Sealed bottle of Beef Extract Peptone Broth powder

Beef Extract Peptone Broth – General Purpose Liquid Medium for Bacterial Culture (250g)

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Classic Beef Extract Peptone Broth for Routine Bacterial Cultivation
This nutrient-rich liquid medium combines high-quality beef extract (5.0g/L) and peptone (10.0g/L) to create an ideal environment for general bacterial growth in suspension. With pH stabilized at 7.3±0.2, it serves as a versatile base for enrichment cultures, industrial fermentation, and basic microbiological research.

Key Applications:

  • 🧫 Broth Cultures: Ideal for bacterial suspension growth and maintenance

  • 🏭 Industrial Processes: Base medium for fermentation and bioproduction

  • 🔬 Research Labs: Reliable for preliminary microbial studies

Technical Advantages:

  • Dual Nutrient System: 5.0g/L beef extract + 10.0g/L peptone

  • Osmotic Balance: 5.0g/L NaCl maintains cell integrity

  • pH-Stable: Maintains 7.3±0.2 throughout growth phases

  • Easy Preparation: 20.0g/L standardized concentration

Preparation Protocol:

  1. Suspend 20.0g in 1L purified water

  2. Warm to 45-50°C for complete dissolution

  3. Autoclave at 121°C for 15 minutes

Complete Formulation (Per Liter):

  • Beef Extract: 5.0g

  • Peptone: 10.0g

  • NaCl: 5.0g

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