Casitone Agar (GA) – Veterinary Biologicals Viability Testing Medium (250g)
Specialized Casitone GA Agar for Microbial Viability Testing in Veterinary Biological Products
Formulated with 15.0g/L pancreatic casein digest and L-cystine enrichment, this agar meets stringent requirements for viability testing in veterinary vaccines and biologics. The optimized pH 7.2±0.2 and dextrose supplementation support robust growth of production strains while maintaining test sensitivity.
Key Applications:
🐄 Vaccine QC: Essential for live bacteria testing in veterinary biologics
🧫 Master Seed Lot Validation: Supports growth of production microorganisms
🧪 Strain Viability: Monitors microbial viability during manufacturing
Critical Technical Features:
✅ Enriched Formula: 15.0g/L casein peptone + 5.0g/L yeast extract
✅ Reducing Agent: 0.5g/L L-cystine for fastidious anaerobes
✅ Energy Source: 5.0g/L dextrose enhances microbial recovery
✅ Validated Sterilization: 116°C for 20-40 minutes protocol
Preparation Protocol:
Suspend 43.0g in 1L purified water
Boil for 1 minute with stirring
Autoclave at 116°C for 20-40 minutes
Critical: Shake during cooling to prevent sedimentation
Pour plates at ≈55°C
Complete Formulation (Per Liter):
Pancreatic Casein Digest: 15.0g
Yeast Extract: 5.0g
L-Cystine: 0.5g
Sodium Chloride: 2.5g
Dextrose: 5.0g
Agar: 15.0g
