Medium 10 (Polymyxin Seed Agar) – 250g for Antibiotic Potency Testing
Medium 10 (Polymyxin Seed Agar) is a specialized solid medium designed for determining antibiotic potency. This 250g bottle provides a high-quality formulation that, when prepared with Tween 80, supports the growth of microorganisms involved in antibiotic potency testing, making it a reliable tool for pharmaceutical and laboratory applications.
Key Features
Primary Use: Specifically formulated for the determination of antibiotic potency. Requires adding 1 vial of Tween 80 per 100ml of medium during preparation.
Formula (per liter): Contains Pancreatic Digest of Casein (17.0g), Papaic digest of Soyabean (3.0g), Sodium chloride (5.0g), Dibasic Potassium Phosphate (2.5g), Dextrose (2.5g), and Agar (12.0g), with a pH of 7.2±0.1 at 25°C.
Storage: Stable at room temperature with a 3-year shelf life, ensuring consistent performance over time.
Preparation Instructions
Measure 42.0g of the medium and add 1 vial (1ml Tween 80) per 100ml of distilled or deionized water (scale proportionally for 1L).
Heat to a boil until fully dissolved, then dispense into containers.
Sterilize at 121°C under high pressure for 15 minutes.
After sterilization, shake well to prevent agar from settling at the bottom. Cool and store for later use.
This Medium 10 is ideal for laboratories requiring accurate antibiotic potency determination through microbiological assays.
