agar plate

Pre-filled TSA Agar Plates – Soybean-Casein Digest Agar for Microbial Monitoring

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Product Overview
Pre-filled TSA (Soybean-Casein Digest Agar) plates are designed for reliable microbial monitoring in cleanrooms, controlled environments, and laboratories. TSA is a general-purpose nutrient medium that supports the growth of a wide range of microorganisms, including bacteria, yeast, and mold. It is widely used for environmental monitoring, settle plate sampling, and general microbial culture applications.

Composition (per liter)

  • Pancreatic digest of casein: 15g

  • Papaic digest of soybean meal: 5g

  • Sodium chloride: 5g

  • Agar: 15g

  • Purified water: 1000ml

  • pH: 7.3 ± 0.2 (at 25°C)

Available Sizes
55mm, 75mm, 90mm, 150mm

Applications

  • Cleanroom microbial monitoring

  • Settle plate sampling in controlled areas

  • General microbial detection and culture experiments

  • Surface contact sampling for environmental hygiene testing

Storage Conditions
Store at 2–25°C, protected from light. Shelf life: 6 months.

Performance Characteristics

  • Appearance: light yellow, smooth surface, free of cracks and visible impurities

  • pH within specification, gel strength 500–750 g/cm²

  • No microbial growth after 5 days incubation at 30–35°C (sterility confirmed)

  • Excellent moisture retention with minimal water loss

  • Supports robust growth of control strains, including Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Candida albicans, and Aspergillus niger

Usage Instructions

  1. Transport the packaged plates to the sampling location. Remove the outer layers step-by-step, opening the final sterile package in the clean area.

  2. For contact plate sampling, press the agar surface gently against the target surface for about 10 seconds, then carefully replace the lid.

  3. Label plates with sampling location, date, and operator information.

  4. Incubate at 30–35°C for at least 48 hours, or follow alternative incubation conditions as required.

  5. Record and count colonies.

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