Standard I Nutrient Broth – 250g | High-Nutrient Bacterial Growth Medium

Premium Enrichment Broth for Robust Bacterial Proliferation

Standard I Nutrient Broth delivers superior growth kinetics with its enhanced peptone profile and balanced electrolytes. Ideal for demanding applications requiring rapid biomass accumulation, this formulation ensures reliable results in both research and quality control settings.

Key Applications:

– Seed culture preparation for fermentation

– Antibiotic susceptibility testing

– Bacterial suspension for vaccine production

– Environmental water sample enrichment

– Diagnostic specimen amplification

Critical Advantages:

✓ Accelerated Growth – 15g/L peptone supports 2X faster doubling time

✓ Universal Compatibility – Excellent for Gram-positive & Gram-negative bacteria

✓ Optical Clarity – Low particulate for turbidity measurements

✓ Rapid Solubility – Dissolves in ≤3 minutes at 45°C

✓ ISO-Compliant – Meets pharmacopeia standards

Formula Composition (per liter):

– Peptone: 15.0g

– Yeast Extract: 3.0g

– Sodium Chloride: 6.0g

– D(+)Glucose: 1.0g

– Final pH: 7.5 ± 0.2 (25°C)

Preparation Protocol:

1. Suspend 25.0g in 1L distilled water

2. Warm to 45°C with constant stirring

3. Autoclave at 121°C for 15 minutes

For fastidious organisms: Supplement with 5% sterile blood

Quality Validation:

– Growth kinetics certified with E. coli ATCC 25922

– Batch-specific pH stability testing

– Endotoxin ≤10 EU/mL

Store sealed below 30°C. Shelf life: 36 months.