2216E Marine Agar – 250g | Oceanic Bacteria Culture
Authentic Marine Environment Simulation for Bacterial Growth
2216E Marine Agar (Zobell 2216) replicates natural seawater chemistry, providing optimal conditions for cultivating and enumerating saltwater bacteria. This specialized medium is essential for marine microbiology research and environmental monitoring.
Critical Applications:
– Isolation of oceanic & estuarine microorganisms
– Marine biofilm studies
– Aquaculture pathogen monitoring
– Deep-sea sample analysis
– Coral reef ecosystem research
Key Advantages:
✓ True Seawater Composition – Balanced ions mirroring ocean salinity
✓ High-Clarity Gel – Enhanced colony visibility despite mineral content
✓ Standardized Formula – Consistent with Zobell’s original formulation
✓ Rapid Dissolution – Simplified preparation protocol
Formula Composition (per liter):
– Peptone: 5.0g
– Yeast Extract: 1.0g
– Sodium Chloride: 19.45g
– Magnesium Chloride: 8.8g
– Calcium Chloride: 1.8g
– Potassium Chloride: 0.55g
– Ferric Citrate: 0.1g
– Sodium Sulfate: 3.24g
– Sodium Bicarbonate: 0.16g
– Potassium Bromide: 0.08g
– Strontium Chloride: 34mg
– Boric Acid: 22mg
– Sodium Fluoride: 2.4mg
– Ammonium Salt: 1.6mg
– Disodium Phosphate: 8mg
– Sodium Silicate: 4mg
– Agar: 15.0g
– Final pH: 7.6 ± 0.2 (25°C)
Preparation Guide:
1. Suspend 55.2g in 1L distilled water
2. Boil with agitation until complete dissolution
3. Autoclave at 121°C for 15 minutes
4. Swirl gently after sterilization
Note: Minor precipitates may form due to mineral content – this does not affect performance.
Quality Verification:
– Osmolarity validated
– pH stability tested
– Growth promotion certified
Store at 10-30°C in sealed container. Shelf life: 24 months.
