Triple Sugar Iron Agar (TSI) Medium – 250g | For Bacteriological Biochemical Testing

Triple Sugar Iron Agar (TSI) Medium is a specialized microbiological culture medium designed for the biochemical differentiation of gram-negative bacteria, particularly enteric pathogens. This 250g formulation enables reliable detection of carbohydrate fermentation (glucose, lactose, sucrose) and hydrogen sulfide production in clinical, research, and industrial laboratories.

‌Key Applications‌

• Identifies bacterial metabolic profiles via sugar fermentation patterns.

• Detects hydrogen sulfide production and gas formation.

• Standardized for ISO-compliant quality control in food, water, and pharmaceutical testing.

• Essential for Salmonella, Shigella, and other Enterobacteriaceae identification.

‌Composition (per liter)‌

• Peptone: 20.0g

• Beef Extract: 5.0g

• Sodium Chloride: 5.0g

• Lactose: 10.0g

• Sucrose: 10.0g

• Dextrose: 1.0g

• Ferrous Sulfate: 0.2g

• Sodium Thiosulfate: 0.2g

• Phenol Red: 0.025g

• Agar: 12.0g

• pH: 7.3 ± 0.1 (25°C)