Lecithin Tween 80 Agar Base (250g) – Cosmetic Microbial Enumeration Medium
Our Lecithin Tween 80 Nutrient Agar Base is engineered for precise microbial enumeration in cosmetics and personal care products. Specifically formulated to neutralize preservatives and support accurate colony counting per international standards.
Key Applications
💄 Cosmetic Total Viable Count: Compliant with USP<61>, EP 2.6.12, and ISO 21149
🧴 Preservative Neutralization: Neutralizes benzalkonium chloride, cetrimide, and quaternary ammonium compounds
📋 Quality Control: Essential for personal care product manufacturers and testing labs
🔬 TTC-Enhanced Option: Ready for TTC solution addition for improved colony visualization
Technical Specifications
Format: Dehydrated powder
Yield: Prepares 6-7 liters of ready medium
pH Range: 7.1-7.4 at 25°C
Sterilization: Autoclavable (121°C/20 min)
Packaging: Moisture-proof bottle (250g)
Certified Formula (per liter)
Peptone 20.0g
Beef Extract 3.0g
Sodium Chloride 5.0g
Lecithin 1.0g
Agar 15.0g
Requires supplementation with 7g Tween 80 per liter
Preparation Protocol
Dissolve: 44.0g powder in 1L distilled water
Supplement: Add 7g Tween 80
Optional TTC: Add 0.5% TTC solution (1 ampoule/100ml)
Sterilize: Autoclave at 121°C for 20 minutes
Mix: Swirl during cooling to prevent agar sedimentation
